Instrument - Sullivan Lab Aerosol Optical Tweezers
Short name:
AOT
Full name:
Sullivan Lab Aerosol Optical Tweezers
What is being measured:
cavity-enhanced Raman spectrum of optically levitated single particles, particle size, particle morphology, refractive index
Sampling Protocol:
Online
Manufacturer:
N/A
Model:
N/A
Instrument year
:
None specified
Data recording software:
Lightfield
Data analysis software:
Raw data time resolution:
0.1-1 Hz
Analysis data averaging:
Detection limit:
N/A
Sensitivity to temperature (and correction method, if applicable):
:
N/A
Sensitivity to relative humidity (and correction method, if applicable):
:
relative humidity is controlled and measured but doesn't affect ability of the instrument to collect data
Sampling method:
liquid solution is atomized and a single droplet is trapped in a single-beam gradient force optical trap (532 nm)
Sample preparation method:
Sample residence time (chamber to instrument) (seconds):
Length of tubing (cm):
Instrument flow rate:
Tubing inner diameter:
Tubing material:
Chemical identification method:
We identify chemicals based off their Raman shift.
Data analysis method:
We use a custom MATLAB algorithm to identify whispering gallery modes (WGMs) in the cavity-enhanced Raman spectrum, which give information about particle morphology, size, and refractive index.
Quantification method:
Raw signal is denoised and peak area is used for external calibration of concentration.
Calibration method:
Wavelength calibration is performed using a Princeton Instruments Ne/Ar light source and the IntelliCal algorithm built into the Lightfield software. Broad calibration is chosen to work over the entire spectral range.
Calibration drift estimate:
Calibration schedule:
Uncertainty estimation method:
Uncertainty arises from noise in the spectrum and is quantified using the custom MATLAB algorithm.
Known interferences:
Link to supplemental information:
https://www.tandfonline.com/doi/full/10.1080/02786826.2016.1224317
Additional notes:
Please note that this instrument provides real-time data from optically levitated single droplets.
Measurement uncertainty:
-
Measurement units:
-