Instrument - Sullivan Lab Aerosol Optical Tweezers
Sullivan Lab Aerosol Optical Tweezers
What is being measured:
cavity-enhanced Raman spectrum of optically levitated single particles, particle size, particle morphology, refractive index
Data recording software:
Data analysis software:
Raw data time resolution:
Analysis data averaging:
Sensitivity to temperature (and correction method, if applicable):
Sensitivity to relative humidity (and correction method, if applicable):
relative humidity is controlled and measured but doesn't affect ability of the instrument to collect data
liquid solution is atomized and a single droplet is trapped in a single-beam gradient force optical trap (532 nm)
Sample preparation method:
Sample residence time (chamber to instrument) (seconds):
Length of tubing (cm):
Instrument flow rate:
Tubing inner diameter:
Chemical identification method:
We identify chemicals based off their Raman shift.
Data analysis method:
We use a custom MATLAB algorithm to identify whispering gallery modes (WGMs) in the cavity-enhanced Raman spectrum, which give information about particle morphology, size, and refractive index.
Raw signal is denoised and peak area is used for external calibration of concentration.
Wavelength calibration is performed using a Princeton Instruments Ne/Ar light source and the IntelliCal algorithm built into the Lightfield software. Broad calibration is chosen to work over the entire spectral range.
Calibration drift estimate:
Uncertainty estimation method:
Uncertainty arises from noise in the spectrum and is quantified using the custom MATLAB algorithm.
Link to supplemental information:
Please note that this instrument provides real-time data from optically levitated single droplets.